• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Egyptian Poultry Science Journal
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 45 (2025)
Volume Volume 44 (2024)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 43 (2023)
Volume Volume 42 (2022)
Volume Volume 41 (2021)
Volume Volume 40 (2020)
Volume Volume 39 (2019)
Volume Volume 38 (2018)
Volume Volume 37 (2017)
Volume Volume 36 (2016)
Volume Volume 35 (2015)
Volume Volume 34 (2014)
Khattab, A., Khalil, M., iraqi, M., amin, E., EL Nagar, A. (2024). MOLECULAR POLYMORPHISM OF MYOSTATIN GENE FOR GROWTH TRAITS IN CROSSBREEDING EXPERIMENT INVOLVING APRI AND MOSHTOHOR RABBIT LINES. Egyptian Poultry Science Journal, 44(1), 15-29. doi: 10.21608/epsj.2024.347265
Abdelwahab H Khattab; Maher Khalil; mahmoud Maghraby iraqi; emad amin; Ayman G. EL Nagar. "MOLECULAR POLYMORPHISM OF MYOSTATIN GENE FOR GROWTH TRAITS IN CROSSBREEDING EXPERIMENT INVOLVING APRI AND MOSHTOHOR RABBIT LINES". Egyptian Poultry Science Journal, 44, 1, 2024, 15-29. doi: 10.21608/epsj.2024.347265
Khattab, A., Khalil, M., iraqi, M., amin, E., EL Nagar, A. (2024). 'MOLECULAR POLYMORPHISM OF MYOSTATIN GENE FOR GROWTH TRAITS IN CROSSBREEDING EXPERIMENT INVOLVING APRI AND MOSHTOHOR RABBIT LINES', Egyptian Poultry Science Journal, 44(1), pp. 15-29. doi: 10.21608/epsj.2024.347265
Khattab, A., Khalil, M., iraqi, M., amin, E., EL Nagar, A. MOLECULAR POLYMORPHISM OF MYOSTATIN GENE FOR GROWTH TRAITS IN CROSSBREEDING EXPERIMENT INVOLVING APRI AND MOSHTOHOR RABBIT LINES. Egyptian Poultry Science Journal, 2024; 44(1): 15-29. doi: 10.21608/epsj.2024.347265

MOLECULAR POLYMORPHISM OF MYOSTATIN GENE FOR GROWTH TRAITS IN CROSSBREEDING EXPERIMENT INVOLVING APRI AND MOSHTOHOR RABBIT LINES

Article 2, Volume 44, Issue 1, March 2024, Page 15-29  XML PDF (813.85 K)
Document Type: Original Article
DOI: 10.21608/epsj.2024.347265
View on SCiNiTO View on SCiNiTO
Authors
Abdelwahab H Khattab1; Maher Khalilorcid 2; mahmoud Maghraby iraqiorcid 3; emad amin4; Ayman G. EL Nagarorcid 5
1Department of Animal and Poultry Breeding, Animal and Poultry Production Division, Desert Research Center, Ministry of Agriculture, El-Matareya 11753, Cairo, Egypt.
2Department of Animal Production, Faculty of Agriculture, Benha University
3Animal production department, faculty of Agriculture, Benha University, Egypt
4Desert Rese. Center, Ministry of Agric., Egypt
5Department of Animal Production, Faculty of Agriculture, Benha University, Egypt
Abstract
Five years crossbreeding experiment was preformed involving two synthetic rabbit lines of APRI (A) and Moshtohor (M) where bucks of APRI line and does of Moshtohor line were used to produce F1 crossbreds (½A½M), followed by inter-se mating to obtain F2 crossbreds (½A½M)2. PCR-RFLP technique was used to detect the SNP genotyping of MSTN gene in APRI and Moshtohor lines and their progenies of F1 and F2 crossbreds and to detect the molecular associations between SNP genotypes of MSTN candidate gene and growth traits using FspBI restriction enzyme. Two genotypes of MSTN gene were detected (TT and TA) in each separate genetic group. The variance components estimated by Gibbs Sampling were used to solve the mixed model equations using the PEST software, getting the solutions for genetic groups effects along with the solutions for other fixed effects. Then, the generalized least-square means (GLM) for the two genotypes of the MSTN gene were estimated using the PEST software. The GLM obtained for body weights at 4, 6, 8, 10 and 12 weeks of age in both crosses of ½A½M and (½A½M)2 were higher than those of their parental lines of APRI and Moshtohor. But, APRI-line rabbits were nearly similar in body weights to Moshtohor line rabbits at 8, 10 and 12 weeks of age. The effective numbers of alleles (Ne) were 1.26, 1.08, 1.11 and 1.37 in APRI line, Moshtohor line, ½A½M and (½A½M)2 crosses, respectively. High degrees of similarity were detected between the numbers of expected and observed genotypes in all genetic groups and therefore these genetic groups were in Hardy-Weinberg equilibrium (HWE) for MSTN gene. The values of polymorphic information content (PIC) were low and ranged from 0.073 in Moshtohor line to 0.235 in (½A½M)2 cross. The genotypic frequencies of TT genotype of MSTN gene were high in all genetic groups (0.68 to 0.92). The allelic frequency recorded for T allele was much higher than for A allele. The heterozygosis for MSTN gene in all genetic groups were low or moderate, ranging from 0.079 to 0.323 for the observed heterozygosity (HO) and 0.076 to 0.271 for the expected heterozygosity (He). The reductions in heterozygosity due to inbreeding (FIS) in all genetic groups were low. In different genetic groups, both TT and TA genotypes of MSTN gene were found to be significantly associated with most body weights and gains at different ages. In APRI line and (½A½M)2 cross, TT genotype was positively associated with an increase in most body weights. In Moshtohor and ½A½M cross, TA genotype was positively associated with heavy body weights. High means for TT and TA genotypes of MSTN gene in ½A½M and (½A½M)2 crosses were recorded and showing strong positive associations of both TT and TA genotypes of MSTN gene with most body weights and gains.
 
Keywords
Rabbits; crossbreeding; growth; Myostatin gene; polymorphism; PCR-RFLP
Statistics
Article View: 162
PDF Download: 258
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.