CHARACTERIZATION OF SELECTED JAPANESE QUAIL AND RANDOMBRED CONTROL LINES IN THE 4th GENERATION BASED ON PRODUCTIVE PERFORMANCE AND RAPD-PCR ANALYSIS

doi:10.21608/epsj.2014.5370

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	CHARACTERIZATION OF SELECTED JAPANESE QUAIL AND RANDOMBRED CONTROL LINES IN THE 4th GENERATION BASED ON PRODUCTIVE PERFORMANCE AND RAPD-PCR ANALYSIS
Article 4, Volume 34, Issue 4, December 2014, Page 869-893
Document Type: Original Article
DOI: 10.21608/epsj.2014.5370
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Abstract
Productive performance and RAPD analysis were used to find the genetic variations and relatedness among two selected, maternal (line1) and long shank length (line3), and randombred (line2) males and females of Japanese quail in the 4th generation of selection. Body weight (BW) and shank length (SL) were measured at 1, 7, 14, 21, 28 and 35 days of age and BW at sexual maturity. Age at first egg (AFE), Age at 10 eggs (Age10), Age at 30 eggs (Age30), number of days needed to produce the first 10 eggs (DN10), number of days needed to produce the first 30 eggs (DN30), egg mass of the first 10 eggs (EM10) and egg mass of the first 30 eggs (EM30) were recorded individually for each female. Individual blood samples were collected from 60 birds [20 birds (10 males and 10 females/line)] to extract DNA. RAPD-PCR amplification was performed using 10 random primers that succeeded to generate informative polymorphic bands. The results indicated that, line3 had higher BW and SL than line1 and line2 at all studied ages except at one day old, while the line2 had the significant lowest values at all ages. The line1 matured at earlier age than the line2 and line3, and had shorter DN10 and DN30. Also, line1 had lower Age10 and Age30 than the line2 and line3, and had heavier EM10 and EM30 than the line2 and line3.
Keywords
Quail; selection; maternal; Shank; Randombred; RAPD analysis; similarity


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