(2017). EFFECT OF ASCORBIC ACID INJECTION IN PRE-INCUBATED HY-LINE LAYER EGGS ON HATCHABILITY AND SOME BLOOD AND HEMATOLOGICAL PARAMETERS OF HATCHED CHICKS. Egyptian Poultry Science Journal, 37(4), 1141-1151. doi: 10.21608/epsj.2017.5387
. "EFFECT OF ASCORBIC ACID INJECTION IN PRE-INCUBATED HY-LINE LAYER EGGS ON HATCHABILITY AND SOME BLOOD AND HEMATOLOGICAL PARAMETERS OF HATCHED CHICKS". Egyptian Poultry Science Journal, 37, 4, 2017, 1141-1151. doi: 10.21608/epsj.2017.5387
(2017). 'EFFECT OF ASCORBIC ACID INJECTION IN PRE-INCUBATED HY-LINE LAYER EGGS ON HATCHABILITY AND SOME BLOOD AND HEMATOLOGICAL PARAMETERS OF HATCHED CHICKS', Egyptian Poultry Science Journal, 37(4), pp. 1141-1151. doi: 10.21608/epsj.2017.5387
EFFECT OF ASCORBIC ACID INJECTION IN PRE-INCUBATED HY-LINE LAYER EGGS ON HATCHABILITY AND SOME BLOOD AND HEMATOLOGICAL PARAMETERS OF HATCHED CHICKS. Egyptian Poultry Science Journal, 2017; 37(4): 1141-1151. doi: 10.21608/epsj.2017.5387
EFFECT OF ASCORBIC ACID INJECTION IN PRE-INCUBATED HY-LINE LAYER EGGS ON HATCHABILITY AND SOME BLOOD AND HEMATOLOGICAL PARAMETERS OF HATCHED CHICKS
The present work was carried out to study the effect of ascorbic acid (AsA) injection into fertile Hy-line layer eggs on hatchability, mortality and some blood and hematological parameters of hatched chicks. Three hundred Hy-line layer eggs were divided into four equal groups with 75 eggs for each group with three replicate (25 fertile eggs for each replicate). The first group was injected, pre-incubation, in the air cell by 50 μl deionized water and served as a sham control. While the second, third and fourth groups were injected in the air cell with either 2, 4 or 6 % ascorbic acid. dissolved in 50 μl deionized water and served as AsA-treated groups. Eggs were incubated at 37.5°C and 60 % relative humidity during the first 19th days of incubation. Eggs were turned automatically every 2 hour until the 19th day. At the 10th day of incubation, eggs were examined by light candling to remove infertile eggs . All eggs were transferred to the hatchery at the end of the 19th day of incubation, and placed in hatching trays at 37°C temperature and 70-75 % relative humidity until hatching. hatchability rate, hatching and hatched chick's body weight unlike mortality were significantly higher in the 6 % AsA groups than all the AsA groups or the control group. Hemoglobin concentration was significantly (p<0.05) higher in the the 4 % AsA group and the 6 % AsA group (p<0.05) as compared to the 2 % AsA or control groups. However there was no significant difference in the cholesterol concentration between the control group and all the AsA groups. Glucose and triiodothyronin concentrations were significantly (p<0.05) higher in the 6 % AsA group than the 2 % AsA or control groups. In conclusion, our study suggests injecting 50 microliters of 6 % ascorbic acid reduced embryonic mortality and improvd hatchability and hatched chicks’body weight.
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