El-Tahawy, W. (2025). GENETIC IMPROVEMENT OF BANDARA DEVELOPED CHICKEN STRAIN USING SPERM – MEDIATED GENE TRANSFER W. S. EL-Tahawy;1 A.E.Abd Al Hamid ;1Ghanem H.H.; 2A.N.Nawar2 and O.M.Aly2 1Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Egypt 2Animal Production Research Institute, Agriculture Research Center, Giza, Egypt Corresponding author: Waleed salah EL-Tahawy;E mail:waleed.eltahawy@agr.dmu.edu.eg. Egyptian Poultry Science Journal, (), -. doi: 10.21608/epsj.2025.80621.1167
waleed salah El-Tahawy. "GENETIC IMPROVEMENT OF BANDARA DEVELOPED CHICKEN STRAIN USING SPERM – MEDIATED GENE TRANSFER W. S. EL-Tahawy;1 A.E.Abd Al Hamid ;1Ghanem H.H.; 2A.N.Nawar2 and O.M.Aly2 1Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Egypt 2Animal Production Research Institute, Agriculture Research Center, Giza, Egypt Corresponding author: Waleed salah EL-Tahawy;E mail:waleed.eltahawy@agr.dmu.edu.eg". Egyptian Poultry Science Journal, , , 2025, -. doi: 10.21608/epsj.2025.80621.1167
El-Tahawy, W. (2025). 'GENETIC IMPROVEMENT OF BANDARA DEVELOPED CHICKEN STRAIN USING SPERM – MEDIATED GENE TRANSFER W. S. EL-Tahawy;1 A.E.Abd Al Hamid ;1Ghanem H.H.; 2A.N.Nawar2 and O.M.Aly2 1Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Egypt 2Animal Production Research Institute, Agriculture Research Center, Giza, Egypt Corresponding author: Waleed salah EL-Tahawy;E mail:waleed.eltahawy@agr.dmu.edu.eg', Egyptian Poultry Science Journal, (), pp. -. doi: 10.21608/epsj.2025.80621.1167
El-Tahawy, W. GENETIC IMPROVEMENT OF BANDARA DEVELOPED CHICKEN STRAIN USING SPERM – MEDIATED GENE TRANSFER W. S. EL-Tahawy;1 A.E.Abd Al Hamid ;1Ghanem H.H.; 2A.N.Nawar2 and O.M.Aly2 1Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Egypt 2Animal Production Research Institute, Agriculture Research Center, Giza, Egypt Corresponding author: Waleed salah EL-Tahawy;E mail:waleed.eltahawy@agr.dmu.edu.eg. Egyptian Poultry Science Journal, 2025; (): -. doi: 10.21608/epsj.2025.80621.1167
GENETIC IMPROVEMENT OF BANDARA DEVELOPED CHICKEN STRAIN USING SPERM – MEDIATED GENE TRANSFER W. S. EL-Tahawy;1 A.E.Abd Al Hamid ;1Ghanem H.H.; 2A.N.Nawar2 and O.M.Aly2 1Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Egypt 2Animal Production Research Institute, Agriculture Research Center, Giza, Egypt Corresponding author: Waleed salah EL-Tahawy;E mail:waleed.eltahawy@agr.dmu.edu.eg
Articles in Press, Accepted Manuscript, Available Online from 19 May 2025
ANIMAL AND POULTRY PRODUCTION .Fac.of Agric. Damanhour Unvi.
Abstract
The experimental was aimed to improve genetic of Bandara Local chicken strain using sperm to insert Growth hormone gene (cGH) from (cobb 500) broiler strain by(SMGT) method sperm-mediated gene transfer technique. This study investigated for two generation. Total RNA was extracted from chicken liver tissue and the cDNA was successfully prepared.PCR Products GH, mRNA normal Length 810bp for transgenic chicken Bandarah chicken which treat by SMGT method, the same result in first and second generation There were no significant difference between method or generations, the averages of fertility percentage were 89.84 and 91.27 % for SMGT and control. The highest insignificant values were found for hatchability of fertile and total eggs percentage in the control line at the first generation 95.64% and 88.49%, respectively. The method of transferring was decreased the ASM at the second generation by 35.01 d. Egg number which produced during the first 90 days from SMGT method significantly increased compared with the control. There was highly significant difference between method found, the SMGT method had the heaviest egg weight 49.20 g followed by control one 48.22 g. SMGT is an efficient method that will hopefully facilitate the implementation of strategies for securing the benefits that can be expected to arise from the introduction of transgenic chicken. Chicken cGH gene was effect in egg productive traits and reproductive traits and moved from the first generation to the second generation with the same shape and increased the effect. This method will save time to improve egg productive traits and reproductive traits.
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